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GrainGenes Marker Report: Bmac299

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Locus	Bmac299
Type	Microsatellite
Synonym	Synonym Bmac0299
Probe	Bmac299
Possible Orthologs	Bmac0299
Probe	Bmac299
Locus	Bmac0299 Bmac299
Synonym	Other name Bmac0299
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AC)15 Quality = 4: Ladders of bands that are at equal intensity, but may contain scoreable weak products
Type	PCR SSR
PCR primers	5' ATAGATGGTAATCATAAGAACAGG 3' 5' ACTATGACTAGTCGTTTCCACA 3'
SSR size	181
Amplification Conditions	1 cycle of 3 mins @ 94C, 1 min @ 66C, 1 min @ 72C, 5 cycles of 30 secs @ 94C, 30 secs @ 65C (decreasing 1C per cycle), 30 secs @ 72C, 24 cycles of 30 secs @ 94C, 30 secs @ 60C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare