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GrainGenes Marker Report: Bmac303

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Locus	Bmac303
Type	Microsatellite
Synonym	Synonym Bmac0303
Chromosome	5H
Map	Hordeum-Consensus2006-DArT-5H 48.8 Hordeum-SxM-SSR-5H 63.1
	[ Show Nearby Loci ]
Map Data	Barley, Consensus 2006, DArT Barley, Steptoe x Morex, SSR
Probe	Bmac303
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206. Li JZ et al. (2003) Development and genetic mapping of 127 new microsatellite markers in barley Theoretical and Applied Genetics 107:1021-1027.
Remark	Number of populations contributing to the placement of Bmac303 in the Barley, Consensus 2006 DArT maps = 1
Possible Orthologs	Bmac0303 Bmac0303a Bmac0303b Bmac0303c Bmac0303e
Probe	Bmac303
Locus	Bmac0303 [ Show all 6 ]
Synonym	Other name Bmac0303
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AG)13(AC)21 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.68
Type	PCR SSR
PCR primers	5' CCTCCAAGATTAGATCTCTCTC 3' 5' CCGTATATTTAAGAAATGGTGA 3'
SSR size	138
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare