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GrainGenes Marker Report: Bmac31b

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Locus	Bmac31b
Type	Microsatellite
Chromosome	7H
Map	Barley-consensus-2003-7H 66.7
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Map Data	Barley consensus 2003
Probe	Bmac31
Data Source	Langridge, Peter J.
Possible Orthologs	Bmac0031 Bmac31 Bmac31a
Probe for Locus	Bmac31
Locus	Bmac0031 Bmac31 Bmac31a Bmac31b
Synonym	Other name Bmac0031
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AC)28 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.37
Type	PCR SSR
PCR primers	5' AGAGAAAGAGAAATGTCACCA 3' 5' ATACATCCATGTGAGGGC 3' AGAGAAAGAGAAATGTCACCA ATACATCCATGTGAGGGC
SSR size	175
Amplification Conditions	1 cycle of 3 mins @ 94C, 1 min @ 66C, 1 min @ 72C, 5 cycles of 30 secs @ 94C, 30 secs @ 65C (decreasing 1C per cycle), 30 secs @ 72C, 24 cycles of 30 secs @ 94C, 30 secs @ 60C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare