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GrainGenes Marker Report: Bmac32

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Locus	Bmac32
Type	Microsatellite
Synonym	Synonym Bmac0032
Chromosome	1H
Map	Barley-consensus-2003-1H 31.9 Hordeum-Consensus2006-DArT-1H 77.6 Hordeum-SxM-SSR-1H 81.7
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Map Data	Barley consensus 2003 Barley, Consensus 2006, DArT Barley, Steptoe x Morex, SSR
Probe	Bmac32
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206. Li JZ et al. (2003) Development and genetic mapping of 127 new microsatellite markers in barley Theoretical and Applied Genetics 107:1021-1027.
Data Source	Langridge, Peter J.
Remark	Number of populations contributing to the placement of Bmac32 in the Barley, Consensus 2006 DArT maps = 2
Possible Orthologs	Bmac0032
Probe	Bmac32
Locus	Bmac0032 Bmac32
Synonym	Other name Bmac0032
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AC)7T(CA)15(AT)9 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.61
Type	PCR SSR
PCR primers	5' CCATCAAAGTCCGGCTAG 3' 5' GTCGGGCCTCATACTGAC 3'
SSR size	215
Amplification Conditions	1 cycle of 3 mins @ 94C, 1 min @ 66C, 1 min @ 72C, 5 cycles of 30 secs @ 94C, 30 secs @ 65C (decreasing 1C per cycle), 30 secs @ 72C, 24 cycles of 30 secs @ 94C, 30 secs @ 60C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare