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GrainGenes Marker Report: Bmac40

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Locus	Bmac40
Type	Microsatellite
Synonym	Synonym Bmac0040
Chromosome	6H
Map	Hordeum-Consensus2006-DArT-6H 68.2 Hv-HNxH602-6H 179.6
	[ Show Nearby Loci ]
Map Data	Barley, Consensus 2006, DArT Barley, HN x H602, Sato2009 Barley, Steptoe x Morex, SSR
Species	Hordeum vulgare Hordeum vulgare subsp. spontaneum
Probe	Bmac40
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117. Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Remark	Number of populations contributing to the placement of Bmac40 in the Barley, Consensus 2006 DArT maps = 1
Possible Orthologs	Bmac0040 Bmac0040a Bmac0040b
Probe	Bmac40
Locus	Bmac0040 Bmac40 Bmac0040b Bmac0040a
Synonym	Other name Bmac0040
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AC)20 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products Diversity Index (DI) = 0.89
Type	PCR SSR
PCR primers	5' AGCCCGATCAGATTTACG 3' 5' TTCTCCCTTTGGTCCTTG 3'
SSR size	236
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare