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GrainGenes Marker Report: Bmac577

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Locus	Bmac577
Type	Microsatellite
Synonym	Synonym Bmac0577
Probe	Bmac577
Possible Orthologs	Bmac0577
Probe	Bmac577
Locus	Bmac0577 Bmac577
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AC)12 Quality = 4: Ladders of bands that are at equal intensity, but may contain scoreable weak products
Type	PCR SSR
PCR primers	5' TCATACAGAAGCCCACACAG 3' 5' TGCATGTTCATTCTAGACAGG 3'
SSR size	146
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare