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GrainGenes Marker Report: Bmac582

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Locus	Bmac582
Type	Microsatellite
Synonym	Synonym Bmac0582
Probe	Bmac582
Possible Orthologs	Bmac0582
Probe	Bmac582
Locus	Bmac0582 Bmac582
Synonym	Other name Bmac0582
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AC)12 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products
Type	PCR SSR
PCR primers	5' GCCACATATGCACCCTAGTG 3' 5' CATGGGGTAGTTTGTGCCTT 3'
SSR size	170
Amplification Conditions	1 cycle of 3 mins @ 94C, 1 min @ 66C, 1 min @ 72C, 5 cycles of 30 secs @ 94C, 30 secs @ 65C (decreasing 1C per cycle), 30 secs @ 72C, 24 cycles of 30 secs @ 94C, 30 secs @ 60C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare