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GrainGenes Marker Report: Bmac63

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Locus	Bmac63
Type	Microsatellite
Synonym	Synonym Bmac0063
Probe	Bmac63
Possible Orthologs	Bmac0063
Probe	Bmac63
Locus	Bmac0063 Bmac63
Synonym	Other name Bmac0063
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AC)14 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products
Type	PCR SSR
PCR primers	5' AAACATGCACGTCACCTAC 3' 5' ACAACCACTGCATGAACAT 3'
SSR size	125
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare