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GrainGenes Marker Report: Bmac84

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Locus	Bmac84
Type	Microsatellite
Synonym	Synonym Bmac0084
Probe	Bmac84
Possible Orthologs	Bmac0084
Probe	Bmac84
Locus	Bmac0084 Bmac84
Synonym	Other name Bmac0084
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AC)13 Quality = 2: Single product, relatively strong stutter bands Null in H. spontaneum Canada Park.
Type	PCR SSR
PCR primers	5' CTTGTGCCCTTTGATGCAC 3' 5' CATAACTTGAGGATGTGTGTGACA 3'
SSR size	173
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare