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GrainGenes Marker Report: Bmac90

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Locus	Bmac90
Type	Microsatellite
Synonym	Synonym Bmac0090
Chromosome	1H
Map	Barley-consensus-2003-1H 34.4 Hordeum-Consensus2006-DArT-1H 63.9
	[ Show Nearby Loci ]
Map Data	Barley consensus 2003 Barley, Consensus 2006, DArT Barley, Steptoe x Morex, SSR
Probe	Bmac90
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Data Source	Langridge, Peter J.
Remark	Number of populations contributing to the placement of Bmac90 in the Barley, Consensus 2006 DArT maps = 4
Possible Orthologs	Bmac0090
Probe	Bmac90
Locus	Bmac0090 Bmac90
Synonym	Other name Bmac0090
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AC)20 Quality = 2: Single product, relatively strong stutter bands
Type	PCR SSR
PCR primers	5' ACATCAACCCTCCTGCTC 3' 5' CCGCACATAGTGGTTACATC 3' ACATCAACCCTCCTGCTC CCGCACATAGTGGTTACATC
SSR size	221
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare