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GrainGenes Marker Report: Bmac96

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Locus
Bmac96
Type
Microsatellite
Synonym
SynonymBmac0096
Chromosome
5H
Map
Barley-consensus-2003-5H33.6
Hordeum-Consensus2006-DArT-5H51
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Map Data
Barley consensus 2003
Barley, Consensus 2006, DArT
Probe
Bmac96
Reference
ReferenceWenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Data Source
Langridge, Peter J.
Remark
Number of populations contributing to the placement of Bmac96 in the Barley, Consensus 2006 DArT maps = 1
Possible Orthologs
Bmac0096



Probe
Bmac96
Locus
Bmac0096
Bmac96
Synonym
Other nameBmac0096
Reference
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks
Insert contains repeat (AT)6(AC)16
Quality = 2: Single product, relatively strong stutter bands
Diversity Index (DI) = 0.74
Type
PCR
SSR
PCR primers
5' GCTATGGCGTACTATGTATGGTTG 3'
5' TCACGATGAGGTATGATCAAAGA 3'
SSR size
173
Amplification Conditions
1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare