Query (optional)   in Class  

GrainGenes Marker Report: Bmag006

[Submit comment/correction]

Locus
Bmag006
Type
Microsatellite
Chromosome
3H
Map
Hordeum-Zhedar2-FHB-QTL-3H57.2
[ Show Nearby Loci ]
Map Data
Barley, Zhedar2, FHB QTL
Probe
Bmag6
Possible Orthologs
Bmag0006
Bmag6



Probe for Locus
Bmag6
Locus
Bmag0006
Bmag6
Bmag006
Synonym
Other nameBmag0006
Bmag006
Reference
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (AG)17
Quality = 2: Single product, relatively strong stutter bands
Diversity Index (DI) = 0.64
Type
PCR
SSR
PCR primers
5' TTAAACCCCCCCCCTCTAG 3'
5' TGCAGTTACTATCGCTGATTTAGC 3'
SSR size
174
Amplification Conditions
1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare