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GrainGenes Marker Report: Bmag0110

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Locus	Bmag0110
Type	Microsatellite
Chromosome	7H
Map	Barley, B73xCPI-7H 87 Barley-ConsensusFHB-7H 77.8 Hordeum-FxS-FHB-QTL-7H 131.1
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Map Data	Barley, B73xCPI Barley, Consensus 2023, FHB Barley, FxS, FHB QTL
Probe	Bmag0110 Bmag110
Reference	Mesfin A et al. (2003) Quantitative trait loci for fusarium head blight resistance in barley detected in a two-rowed by six-rowed population Crop Science 43:307-318.
Possible Orthologs	Bmag110 Bmag0110a Bmag110A Bmag0110b
Probe for Locus	Bmag110
Locus	Bmag0110 Bmag0110b Bmag0110a Bmag110 Bmag110A
Synonym	Other name Bmag0110
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AG)15 Quality = 2: Single product, relatively strong stutter bands
Type	PCR SSR
PCR primers	5' ACGAGGAGGGACTAGTACAC 3' 5' CCAACTATATTAACAAGGCTCA 3'
SSR size	145
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare