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GrainGenes Marker Report: Bmag0113

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Locus	Bmag0113
Type	Microsatellite
Chromosome	5H
Map	Hordeum-Pillen-5H 62
	[ Show Nearby Loci ]
Map Data	Barley, AxHs/QTL
Probe	Bmag113
Possible Orthologs	Bmag113 [ Show all 16 ]
Probe for Locus	Bmag113
Locus	Bmag0113c [ Show all 17 ]
Synonym	Other name Bmag0113
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AG)18 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products
Type	PCR SSR
PCR primers	5' GGAATCTTCTGGAACGTC 3' 5' TTAAGAAGATCATTGTATTGAAGA 3'
SSR size	153
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare