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GrainGenes Marker Report: Bmag0173

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Locus	Bmag0173
Type	Microsatellite
Synonym	Synonym Bmag173
Chromosome	6 (6H) 6H
Map	Barley, B73xCPI-6H 47.8 [ Show all 12 ]
	[ Show Nearby Loci ]
QTL with Locus Marker	MetaQ.FHB_6H_10 MetaQ.FHB_6H_9 MetaQ.HD_6H_5 QFhs.umn-6H .2
Map Data	Barley, Abiotic QTL Consensus [ Show all 12 ]
Species	Hordeum spontaneum Hordeum vulgare
Probe	Bmag0173 Bmag173
Reference	Abu Qamar M et al. (2008) A region of barley chromosome 6H harbors multiple major genes associated with net type net blotch resistance. Theoretical and Applied Genetics 117:1261-1270. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527. Mesfin A et al. (2003) Quantitative trait loci for fusarium head blight resistance in barley detected in a two-rowed by six-rowed population Crop Science 43:307-318. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Lina x H. spontaneum Insert contains repeat (CT)29 Polymorphic Information Content (PIC) = 0.72 Developing laboratory = SCRI (R. Waugh)
Possible Orthologs	Bmag173
Probe for Locus	Bmag173
Locus	Bmag0173 Bmag173
Synonym	Other name Bmag0173
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (CT)29 Quality = 2: Single product, relatively strong stutter bands Null in H. spontaneum Canada Park. Diversity Index (DI) = 0.72
Type	PCR SSR
PCR primers	5' CATTTTTGTTGGTGACGG 3' 5' ATAATGGCGGGAGAGACA 3'
SSR size	150
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare