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GrainGenes Marker Report: Bmag0306

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Locus	Bmag0306
Type	Microsatellite
Chromosome	4H
Map	Barley, B73xCPI-4H 56.3 Barley-ConsensusFHB-4H 76.8 Hordeum-FxS-FHB-QTL-4H 144.8
	[ Show Nearby Loci ]
Map Data	Barley, B73xCPI Barley, Consensus 2023, FHB Barley, FxS, FHB QTL
Probe	Bmag0306 Bmag306
Reference	Mesfin A et al. (2003) Quantitative trait loci for fusarium head blight resistance in barley detected in a two-rowed by six-rowed population Crop Science 43:307-318.
Possible Orthologs	Bmag306 Bmag0306a Bmag0306b
Probe for Locus	Bmag306
Locus	Bmag0306 Bmag0306b Bmag0306a Bmag306
Synonym	Other name Bmag0306
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AG)16 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products
Type	PCR SSR
PCR primers	5' ATGTACAAGTAGCTATGTGTTTGA 3' 5' CACATCAAGATAACAAGAGAAGA 3'
SSR size	149
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare