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GrainGenes Marker Report: Bmag0705

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Locus	Bmag0705
Type	Microsatellite
Chromosome	3H
Map	Hordeum-Consensus2005-SNP-3H 165.161 Hordeum-LiHs-SNP-3H 157.03 Hordeum-QTLConsensus-Abiotic-3H 165.16 Hordeum-QTLConsensus-Agronomic-3H 165.16
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Map Data	Barley, Abiotic QTL Consensus Barley, Agronomic QTL Consensus Barley, Consensus 2005, SNP Barley, Lina x H.spont, SNP
Probe	EBmag705
Reference	Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527.
Possible Orthologs	EBmag0705 EBmag705 Xmag705
Probe for Locus	EBmag705
Locus	EBmag0705 EBmag705 Bmag0705
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (TC)31 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.8
Type	PCR SSR
PCR primers	5' TCCTGTAGTCCTCTTTGTTTC 3' 5' TATATTACACATGGAGAGAGAGG 3' TCCTGTAGTCCTCTTTGTTTC TATATTACACATGGAGAGAGAGG
SSR size	155
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare