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GrainGenes Marker Report: Bmag0767

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Locus	Bmag0767
Type	Microsatellite
Chromosome	7H
Map	Barley-ConsensusFHB-7H 11.86 Hordeum-FxS-FHB-QTL-7H 9.7
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Map Data	Barley, Consensus 2023, FHB Barley, FxS, FHB QTL
Probe	Bmag767
Reference	Mesfin A et al. (2003) Quantitative trait loci for fusarium head blight resistance in barley detected in a two-rowed by six-rowed population Crop Science 43:307-318.
Probe for Locus	Bmag767
Locus	Bmag0767
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (GA)18 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.69
Type	PCR SSR
PCR primers	5' AACTTACCTTCATATGTTGTGG 3' 5' GAACACTATGATTCCATACGTC 3'
SSR size	151
Amplification Conditions	3 min 94C, 45 cycles of 1 min @94C, 1 min @55C, 2 min @72C, final extension of 10 min @72C. 25 microlitre reactions contained 125 nM of each primer.
Source Species	Hordeum vulgare