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GrainGenes Marker Report: Bmag10

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Locus	Bmag10
Synonym	Synonym Bmag0010a
Probe	Bmag10
Locus	Bmag0010c Bmag0010b Bmag0010a Bmag010
Synonym	Other name Bmag0010
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (CT)19 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products
Type	PCR SSR
PCR primers	5' AGTAGTTCACCCTTGGGGCT 3' 5' AGCACGTGATACATCAAGAACG 3'
SSR size	161
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 69C, 1 min @ 72C, 5 cycles of 30 secs @ 94C, 30 secs @ 68C (decreasing 1C per cycle), 30 secs @ 72C, 24 cycles of 30 secs @ 94C, 30 secs @ 63C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare