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GrainGenes Marker Report: Bmag105

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Locus	Bmag105
Type	Microsatellite
Synonym	Synonym Bmag0105
Chromosome	1H 5H
Map	Barley-consensus-2003-5H 22.7 Hordeum-Consensus2006-DArT-1H 66.2 Hordeum-Zhedar2-FHB-QTL-1H 69.8
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Map Data	Barley consensus 2003 Barley, Consensus 2006, DArT Barley, Zhedar2, FHB QTL
Probe	Bmag105
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Data Source	Langridge, Peter J.
Remark	Number of populations contributing to the placement of Bmag105 in the Barley, Consensus 2006 DArT maps = 1
Possible Orthologs	Bmag0105
Probe	Bmag105
Locus	Bmag0105 Bmag105
Synonym	Other name Bmag0105
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AG)10 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.24
Type	PCR SSR
PCR primers	5' AATCAGACCCATCAGAGGT 3' 5' CCGGTCTCATAGAAATGG 3'
SSR size	108
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare