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GrainGenes Marker Report: Bmag110

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Locus	Bmag110
Type	Microsatellite
Synonym	Synonym Bmag0110a Bmag0110b
Chromosome	7H
Map	Hordeum-Zhedar2-FHB-QTL-7H 76.9
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Map Data	Barley, Zhedar2, FHB QTL
Probe	Bmag110
Possible Orthologs	Bmag0110 Bmag0110a Bmag110A Bmag0110b
Probe	Bmag110
Locus	Bmag0110 Bmag0110b Bmag0110a Bmag110 Bmag110A
Synonym	Other name Bmag0110
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AG)15 Quality = 2: Single product, relatively strong stutter bands
Type	PCR SSR
PCR primers	5' ACGAGGAGGGACTAGTACAC 3' 5' CCAACTATATTAACAAGGCTCA 3'
SSR size	145
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare