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GrainGenes Marker Report: Bmag112

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Locus	Bmag112
Type	Microsatellite
Synonym	Synonym Bmag0112
Chromosome	3H
Map	Barley-consensus-2003-3H 56.7
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Map Data	Barley consensus 2003
Probe	Bmag112
Data Source	Langridge, Peter J.
Possible Orthologs	Bmag0112 Bmag0112.3H Bmag0112.7H Bmag112a Bmag112b
Probe	Bmag112
Locus	Bmag0112 Bmag112 Bmag112a Bmag112b
Synonym	Other name Bmag0112
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AG)18 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products
Type	PCR SSR
PCR primers	5' CCCGTGATATATTAAGATCATG 3' 5' AGGGGGAGATCTTCTCTG 3'
SSR size	196
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare