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GrainGenes Marker Report: Bmag112a

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Locus	Bmag112a
Type	Microsatellite
Chromosome	7H
Map	Hordeum-Consensus2006-DArT-7H 83.6
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Map Data	Barley, Consensus 2006, DArT
Probe	Bmag112
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Remark	Number of populations contributing to the placement of Bmag112a in the Barley, Consensus 2006 DArT maps = 1
Possible Orthologs	Bmag0112 Bmag112 Bmag0112.3H Bmag0112.7H Bmag112b
Probe for Locus	Bmag112
Locus	Bmag0112 Bmag112 Bmag112a Bmag112b
Synonym	Other name Bmag0112
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AG)18 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products
Type	PCR SSR
PCR primers	5' CCCGTGATATATTAAGATCATG 3' 5' AGGGGGAGATCTTCTCTG 3'
SSR size	196
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare