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GrainGenes Marker Report: Bmag120-7H

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Locus
Bmag120-7H
Map
Hv-IxF-Stein-7H116.26
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Map Data
Barley, IxF, Stein2006
Probe
Bmag120
Possible Orthologs
Bmag0120
Bmag120



Probe for Locus
Bmag120
Locus
Bmag0120
Bmag120
Bmag120-7H
Synonym
Other nameBmag0120
Reference
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (AG)15
Quality = 2: Single product, relatively strong stutter bands
Diversity Index (DI) = 0.86
Type
PCR
SSR
PCR primers
5' ATTTCATCCCAAAGGAGAC 3'
5' GTCACATAGACAGTTGTCTTCC 3'
SSR size
230
Amplification Conditions
1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare