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GrainGenes Marker Report: Bmag13

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Locus
Bmag13
Type
Microsatellite
Synonym
SynonymBmag0013
Chromosome
3H
Map
Barley-consensus-2003-3H105.6
Hordeum-Consensus2006-DArT-3H133.5
Hv-Consensus2006-Marcel-3H113.7
Hv-HNxH602-3H231.9
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Map Data
Barley consensus 2003
Barley, Consensus 2006, DArT
Barley, Consensus 2006, Marcel
Barley, HN x H602, Sato2009
Species
Hordeum vulgare
Hordeum vulgare subsp. spontaneum
Probe
Bmag13
Reference
ReferenceSato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
ReferenceWenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Data Source
Langridge, Peter J.
Remark
Number of populations contributing to the placement of Bmag13 in the Barley, Consensus 2006 DArT maps = 3
Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : L94 x Vada; Oregon Wolfe Dom x Rec; Cebada Capa x SusPtrit
Possible Orthologs
Bmag0013
Bmag013



Probe
Bmag13
Locus
Bmag0013
Bmag13
Bmag013
Synonym
Other nameBmag0013
Reference
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks
Insert contains repeat (CT)21
Quality = 4: Ladders of bands that are at equal intensity, but may contain scoreable weak products
Diversity Index (DI) = 0.72
Type
PCR
SSR
PCR primers
5' AAGGGGAATCAAAATGGGAG 3'
5' TCGAATAGGTCTCCGAAGAAA 3'
SSR size
155
Amplification Conditions
1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare