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GrainGenes Marker Report: Bmag135

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Locus	Bmag135
Type	Microsatellite
Chromosome	7H
Map	Barley-consensus-2003-7H 170.1 Hv-Consensus2006-Marcel-7H 147.51
	[ Show Nearby Loci ]
QTL with Locus Marker	QNba-VBDa-7H QNbs-VBDa-7H
Map Data	Barley consensus 2003 Barley, Consensus 2006, Marcel Barley, Steptoe x Morex, SSR
Probe	Bmag135
Data Source	Langridge, Peter J.
Remark	Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : L94 x Vada; Oregon Wolfe Dom x Rec; Steptoe x Morex
Probe	Bmag135
Locus	Bmag0135 Bmag135 Bmag135a Bmag135b
Synonym	Other name Bmag0135 Bmag135
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AG)10GG(AG)12 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.87
Type	PCR SSR
PCR primers	5' ACGAAAGAGTTACAACGGATA 3' 5' GTTTACCACAGATCTACAGGTG 3'
SSR size	161
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare