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GrainGenes Marker Report: Bmag138

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Locus	Bmag138
Type	Microsatellite
Synonym	Synonym Bmag0138a Bmag0138b
Chromosome	4H
Map	Hordeum-IxF-SSR-4H 139.4 Hordeum-Zhedar2-FHB-QTL-4H 157.7
	[ Show Nearby Loci ]
Map Data	Barley, Igri x Franka, SSR Barley, Steptoe x Morex, SSR Barley, Zhedar2, FHB QTL
Probe	Bmag138
Reference	Li JZ et al. (2003) Development and genetic mapping of 127 new microsatellite markers in barley Theoretical and Applied Genetics 107:1021-1027.
Possible Orthologs	Bmag0138 [ Show all 8 ]
Probe	Bmag138
Locus	Bmag0138a [ Show all 6 ]
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AG)6(AG)15 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.62
Type	PCR SSR
PCR primers	5' ACCAGGAGGAATGAGAGAG 3' 5' AATAAACCTTGAGACGATGG 3'
SSR size	169
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare