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GrainGenes Marker Report: Bmag140

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Locus	Bmag140
Type	Microsatellite
Chromosome	2H
Map	Barley-consensus-2003-2H 78 Hv-Consensus2006-Marcel-2H 76.26
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Map Data	Barley consensus 2003 Barley, Consensus 2006, Marcel Barley, Steptoe x Morex, SSR
Probe	Bmag140
Data Source	Langridge, Peter J.
Remark	Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : Steptoe x Morex
Possible Orthologs	Bmag0140
Probe	Bmag140
Locus	Bmag0140 Bmag140
Synonym	Other name Bmag0140
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AG)15 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.35
Type	PCR SSR
PCR primers	5' GCGATCCCATATACTTGTG 3' 5' TCGGATTTAAGTATCTCTGGA 3' GCGATCCCATATACTTGTG TCGGATTTAAGTATCTCTGGA
SSR size	157
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare