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GrainGenes Marker Report: Bmag173

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Locus	Bmag173
Type	Microsatellite
Synonym	Synonym Bmag0173
Chromosome	6H
Map	Hordeum-Consensus2006-DArT-6H 65 Hordeum-Zhedar2-FHB-QTL-6H 78.8
	[ Show Nearby Loci ]
QTL with Locus Marker	QGwe.TaER-6H.1 QHt.TaER-6H.1 QLg.TaER-6H.1 QYld.TaER-6H.1
Map Data	Barley, Consensus 2006, DArT Barley, Zhedar2, FHB QTL
Probe	Bmag173
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Remark	Number of populations contributing to the placement of Bmag173 in the Barley, Consensus 2006 DArT maps = 2
Possible Orthologs	Bmag0173
Probe	Bmag173
Locus	Bmag0173 Bmag173
Synonym	Other name Bmag0173
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (CT)29 Quality = 2: Single product, relatively strong stutter bands Null in H. spontaneum Canada Park. Diversity Index (DI) = 0.72
Type	PCR SSR
PCR primers	5' CATTTTTGTTGGTGACGG 3' 5' ATAATGGCGGGAGAGACA 3'
SSR size	150
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare