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GrainGenes Marker Report: Bmag183

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Locus	Bmag183
Type	Microsatellite
Synonym	Synonym Bmag0183
Chromosome	7H
Map	Barley-consensus-2003-7H 78.6 Hordeum-GxHN-SFNB-7H 21.5
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Map Data	Barley consensus 2003 Barley, GxHN, SFNB
Probe	Bmag183
Data Source	Langridge, Peter J.
Possible Orthologs	Bmag0183
Probe	Bmag183
Locus	Bmag0183 Bmag183
Synonym	Other name Bmag0183
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (CT)12 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products
Type	PCR SSR
PCR primers	5' AGGTTCATCACAAGAAGATAGA 3' 5' ATGTACCAGTAGCTATGTGTTTC 3'
SSR size	146
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare