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GrainGenes Marker Report: Bmag219

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Locus	Bmag219
Type	Microsatellite
Synonym	Synonym Bmag0219
Probe	Bmag219
Possible Orthologs	Bmag0219
Probe	Bmag219
Locus	Bmag0219 Bmag219
Synonym	Other name Bmag0219
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AG)5GG(AG)14 Quality = 5: Bands of unexpected size or additional bands. Very weak bands or no amplification products
Type	PCR SSR
PCR primers	5' ATATTTATGAAACGGTGAAGC 3' 5' GGGTTTATCCTCTGGTCC 3'
SSR size	181
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare