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GrainGenes Marker Report: Bmag222

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Locus	Bmag222
Type	Microsatellite
Synonym	Synonym Bmag0222
Chromosome	5H
Map	Barley-consensus-2003-5H 58.4 Hordeum-Consensus2006-DArT-5H 159.4 Hv-Consensus2006-Marcel-5H 144.14
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Map Data	Barley consensus 2003 Barley, Consensus 2006, DArT Barley, Consensus 2006, Marcel
Probe	Bmag222
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Data Source	Langridge, Peter J.
Remark	Number of populations contributing to the placement of Bmag222 in the Barley, Consensus 2006 DArT maps = 1 Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : L94 x Vada; Oregon Wolfe Dom x Rec
Possible Orthologs	Bmag0222 Bmag222-5H
Probe	Bmag222
Locus	Bmag0222 Bmag222 Bmag222-5H
Synonym	Other name Bmag0222
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AC)9(AG)17 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.6
Type	PCR SSR
PCR primers	5' ATGCTACTCTGGAGTGGAGTA 3' 5' GACCTTCAACTTTGCCTTATA 3'
SSR size	179
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare