GrainGenes Marker Report: Bmag222-5H
Locus
Bmag222-5H
Type
Microsatellite
Map
Hv-DxR-Stein-5H 180.12
[ Show Nearby Loci ]
Map Data
Barley, OWB, Stein2006
Probe
Bmag222
Possible Orthologs
Bmag0222 Bmag222
Probe for Locus
Bmag222
Locus
Bmag0222 Bmag222 Bmag222-5H
Synonym
Other name Bmag0222
Reference
Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (AC)9(AG)17 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.6
Type
PCR SSR
PCR primers
5' ATGCTACTCTGGAGTGGAGTA 3'
5' GACCTTCAACTTTGCCTTATA 3'
SSR size
179
Amplification Conditions
1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare
GrainGenes Marker Report: Bmag222-5H
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