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GrainGenes Marker Report: Bmag223

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Locus	Bmag223
Type	Microsatellite
Synonym	Synonym Bmag0223
Chromosome	5H
Map	Barley-consensus-2003-5H 59.4 [ Show all 6 ]
	[ Show Nearby Loci ]
Map Data	Barley consensus 2003 [ Show all 6 ]
Species	Hordeum vulgare Hordeum vulgare subsp. spontaneum
Probe	Bmag223
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117. Li JZ et al. (2003) Development and genetic mapping of 127 new microsatellite markers in barley Theoretical and Applied Genetics 107:1021-1027.
Data Source	Langridge, Peter J.
Remark	Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : L94 x Vada; Steptoe x Morex; Oregon Wolfe Dom x Rec; Igri x Franka; Cebada Capa x SusPtrit
Possible Orthologs	Bmag0223
Probe	Bmag223
Locus	Bmag0223 Bmag223
Synonym	Other name Bmag0223
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AG)16 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.82
Type	PCR SSR
PCR primers	5' TTAGTCACCCTCAACGGT 3' 5' CCCCTAACTGCTGTGATG 3'
SSR size	127
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare