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GrainGenes Marker Report: Bmag23

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Locus
Bmag23
Type
Microsatellite
Chromosome
3H
Map
Hordeum-Consensus2006-DArT-3H58
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Map Data
Barley, Consensus 2006, DArT
Probe
Bmag23
Reference
ReferenceWenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Remark
Number of populations contributing to the placement of Bmag23 in the Barley, Consensus 2006 DArT maps = 1



Probe
Bmag23
Locus
Bmag0023
Bmag23
Synonym
Other nameBmag0023
Reference
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks
Insert contains repeat (AG)18
Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products
Type
PCR
SSR
PCR primers
5' AACACAGACCTACGGGTC 3'
5' CATGAGATAGATCCAAGCAC 3'
SSR size
137
Amplification Conditions
1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare