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GrainGenes Marker Report: Bmag306

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Locus	Bmag306
Type	Microsatellite
Synonym	Synonym Bmag0306b
Chromosome	4H
Map	Hordeum-Zhedar2-FHB-QTL-4H 104.8
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Map Data	Barley, Zhedar2, FHB QTL
Probe	Bmag306
Possible Orthologs	Bmag0306 Bmag0306a Bmag0306b
Probe	Bmag306
Locus	Bmag0306 Bmag0306b Bmag0306a Bmag306
Synonym	Other name Bmag0306
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AG)16 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products
Type	PCR SSR
PCR primers	5' ATGTACAAGTAGCTATGTGTTTGA 3' 5' CACATCAAGATAACAAGAGAAGA 3'
SSR size	149
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare