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GrainGenes Marker Report: Bmag318

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Locus
Bmag318
Type
Microsatellite
Synonym
SynonymBmag0318a
Chromosome
3H
Map
Hordeum-Consensus2006-DArT-3H62.2
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Map Data
Barley, Consensus 2006, DArT
Probe
Bmag318
Reference
ReferenceWenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Remark
Number of populations contributing to the placement of Bmag318 in the Barley, Consensus 2006 DArT maps = 1
Possible Orthologs
Bmag0318
Bmag0318a
Bmag0318b



Probe
Bmag318
Locus
Bmag0318b
Bmag0318a
Bmag318
Synonym
Other nameBmag0318
Reference
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks
Insert contains repeat (AG)16
Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products
Type
PCR
SSR
PCR primers
5' GATGAGGAGCAAGTAGTTCAC 3'
5' GATGGTAAGGGAAATACATCA 3'
SSR size
173
Amplification Conditions
1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare