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GrainGenes Marker Report: Bmag341a

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Locus	Bmag341a
Type	Microsatellite
Chromosome	2H
Map	Barley-consensus-2003-2H 36.5
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Map Data	Barley consensus 2003
Probe	Bmag341
Data Source	Langridge, Peter J.
Possible Orthologs	Bmag0341 Bmag341 Bmag341b
Probe for Locus	Bmag341
Locus	Bmag0341 Bmag341 Bmag341a Bmag341b
Synonym	Other name Bmag0341
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AG)14 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.62
Type	PCR SSR
PCR primers	5' TCATGGAGACCGTTGTAGT 3' 5' CCACAAGCCTCTGTTCTC 3' TCATGGAGACCGTTGTAGT CCACAAGCCTCTGTTCTC
SSR size	215
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare