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GrainGenes Marker Report: Bmag345b

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Locus	Bmag345b
Type	Microsatellite
Chromosome	4H
Map	Hordeum-Consensus2006-DArT-4H 78.9
	[ Show Nearby Loci ]
Map Data	Barley, Consensus 2006, DArT
Probe	Bmag345
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Remark	Number of populations contributing to the placement of Bmag345b in the Barley, Consensus 2006 DArT maps = 1
Possible Orthologs	Bmag345 Bmag345a
Probe for Locus	Bmag345
Locus	Bmag0345 Bmag345 Bmag345a Bmag345b
Synonym	Other name Bmag0345
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (CT)6AA(CT)9AA(CT)6AA(CT)7AA(CT)7 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.29
Type	PCR SSR
PCR primers	5' ATGAGGAATAACTCAACCAAA 3' 5' AATATATTTTCGATGTCGAGC 3'
SSR size	140
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare