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GrainGenes Marker Report: Bmag350

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Locus	Bmag350
Type	Microsatellite
Synonym	Synonym Bmag0350d
Chromosome	2H
Map	Hordeum-Consensus2006-DArT-2H 79.6
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Map Data	Barley, Consensus 2006, DArT
Probe	Bmag350
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Remark	Number of populations contributing to the placement of Bmag350 in the Barley, Consensus 2006 DArT maps = 1
Possible Orthologs	Bmag0350 Bmag0350a Bmag0350b Bmag0350c Bmag0350d
Probe	Bmag350
Locus	Bmag0350c Bmag0350a Bmag0350b Bmag0350d Bmag350
Synonym	Other name Bmag0350
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AG)20 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products
Type	PCR SSR
PCR primers	5' AGCTAGATCTCTCTGTCTCTGTC 3' 5' AAGAAACACACCACAAAGATT 3'
SSR size	139
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare