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GrainGenes Marker Report: Bmag357

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Locus	Bmag357
Type	Microsatellite
Synonym	Synonym Bmag0357
Probe	Bmag357
Possible Orthologs	Bmag0357
Probe	Bmag357
Locus	Bmag0357 Bmag357
Synonym	Other name Bmag0357
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AG)8G(AG)9 Quality = 2: Single product, relatively strong stutter bands
Type	PCR SSR
PCR primers	5' CTTCTACATCATCCTTGTTGC 3' 5' ATGATCATTGTATTGAAGAGCA 3'
SSR size	146
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare