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GrainGenes Marker Report: Bmag359

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Locus	Bmag359
Type	Microsatellite
Synonym	Synonym Bmag0359
Chromosome	7H
Map	Hordeum-Zhedar2-FHB-QTL-7H 78.2 Hv-HNxH602-7H 155.3
	[ Show Nearby Loci ]
Map Data	Barley, HN x H602, Sato2009 Barley, Zhedar2, FHB QTL
Species	Hordeum vulgare Hordeum vulgare subsp. spontaneum
Probe	Bmag359
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Possible Orthologs	Bmag0359 Bmag359a Bmag359b
Probe	Bmag359
Locus	Bmag0359 Bmag359 Bmag359a Bmag359b
Synonym	Other name Bmag0359
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AG)25 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.52
Type	PCR SSR
PCR primers	5' TGATGACCATTGTATTGAAGA 3' 5' GTTCCTCTCTTAATGATGTGTG 3' TGATGACCATTGTATTGAAGA GTTCCTCTCTTAATGATGTGTG
SSR size	150
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare