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GrainGenes Marker Report: Bmag359b

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Locus	Bmag359b
Type	Microsatellite
Chromosome	7H
Map	Barley-consensus-2003-7H 64.8
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Map Data	Barley consensus 2003
Probe	Bmag359
Data Source	Langridge, Peter J.
Possible Orthologs	Bmag0359 Bmag359 Bmag359a
Probe for Locus	Bmag359
Locus	Bmag0359 Bmag359 Bmag359a Bmag359b
Synonym	Other name Bmag0359
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AG)25 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.52
Type	PCR SSR
PCR primers	5' TGATGACCATTGTATTGAAGA 3' 5' GTTCCTCTCTTAATGATGTGTG 3' TGATGACCATTGTATTGAAGA GTTCCTCTCTTAATGATGTGTG
SSR size	150
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare