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GrainGenes Marker Report: Bmag378

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Locus
Bmag378
Type
Microsatellite
Chromosome
2H
Map
Barley-consensus-2003-2H72.8
Hordeum-Consensus2006-DArT-2H84
Hordeum-Zhedar2-FHB-QTL-2H104.1
Hv-Consensus2006-Marcel-2H76.11
[ Show Nearby Loci ]
QTL with Locus Marker
QHD.dah-2H.1
Map Data
Barley consensus 2003
Barley, Consensus 2006, DArT
Barley, Consensus 2006, Marcel
Barley, Zhedar2, FHB QTL
Probe
Bmag378
Reference
ReferenceWenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Data Source
Langridge, Peter J.
Remark
Number of populations contributing to the placement of Bmag378 in the Barley, Consensus 2006 DArT maps = 2
Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : Oregon Wolfe Dom x Rec
Possible Orthologs
Bmag0378
Bmag0378.2H



Probe
Bmag378
Locus
Bmag0378
Bmag378
Synonym
Other nameBmag0378
Reference
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks
Insert contains repeat (AG)14
Quality = 2: Single product, relatively strong stutter bands
Diversity Index (DI) = 0.79
Type
PCR
SSR
PCR primers
5' CTTTTGTTTCCGTAGCATCTA 3'
5' ATCCAACTATAGTAGCAAAGCC 3'
SSR size
147
Amplification Conditions
1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare