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GrainGenes Marker Report: Bmag384

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Locus	Bmag384
Synonym	Synonym Bmag0384
Chromosome	4H 7H
Map	Hordeum-Consensus2006-DArT-7H 38.5 Hv-Consensus2006-Marcel-4H 57.5
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Map Data	Barley, Consensus 2006, DArT Barley, Consensus 2006, Marcel
Probe	Bmag384
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Remark	Number of populations contributing to the placement of Bmag384 in the Barley, Consensus 2006 DArT maps = 1 Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : L94 x Vada; Oregon Wolfe Dom x Rec; Cebada Capa x SusPtrit
Possible Orthologs	Bmag0384
Probe	Bmag384
Locus	Bmag0384 Bmag384
Synonym	Other name Bmag0384
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AG)18 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.76
Type	PCR SSR
PCR primers	5' TGTGAGTAGTTCACCATAGACC 3' 5' TGCCATTATCATTGTATTGAA 3'
SSR size	116
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare