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GrainGenes Marker Report: Bmag394

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Locus	Bmag394
Type	Microsatellite
Synonym	Synonym Bmag0394
Chromosome	5H
Map	Hordeum-Consensus2006-DArT-5H 57
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Map Data	Barley, Consensus 2006, DArT
Probe	Bmag394
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Remark	Number of populations contributing to the placement of Bmag394 in the Barley, Consensus 2006 DArT maps = 1
Possible Orthologs	Bmag0394
Probe	Bmag394
Locus	Bmag0394 Bmag394
Synonym	Other name Bmag0394
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AG)9CG(AG)4CG(AG)4 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.64
Type	PCR SSR
PCR primers	5' AATTCATCACAACAAGATAGGA 3' 5' AATTGATCTCCCTCTCTCTATG 3'
SSR size	171
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare