GrainGenes Marker Report: Bmag507-7H
Locus
Bmag507-7H
Type
Microsatellite
Map
Hv-SxM-Stein-7H 73.72
[ Show Nearby Loci ]
Map Data
Barley, SxM, Stein2006
Probe
Bmag507
Possible Orthologs
Bmag0500 Bmag500 Bmag507 Xmag500.1 Xmag500.2
Probe for Locus
Bmag507
Locus
Bmag0507 Bmag507 Bmag507-7H
Synonym
Other name Bmag0507
Reference
Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (AG)23 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.69
Type
PCR SSR
PCR primers
5' CAACTAGCATACCCAAGCTATA 3'
5' ACCCCTATTTCAATAATGGA 3'
SSR size
147
Amplification Conditions
1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare
GrainGenes Marker Report: Bmag507-7H
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