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GrainGenes Marker Report: Bmag508

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Locus	Bmag508
Type	Microsatellite
Synonym	Synonym Bmag0508A
Probe	Bmag508
Possible Orthologs	Bmag0508A
Probe	Bmag508
Locus	Bmag0508A Bmag508 Bmag508A
Synonym	Other name Bmag0508 Bmag0508A
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AG)14 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.14
Type	PCR SSR
PCR primers	5' TCTCCGTATATTTAGGAAACG 3' 5' TATCTCCCCCTAGATAGAAGG 3'
SSR size	175
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare