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GrainGenes Marker Report: Bmag518

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Locus	Bmag518
Type	Microsatellite
Synonym	Synonym Bmag0518
Chromosome	2H
Map	Barley-consensus-2003-2H 65.6 Hordeum-Consensus2006-DArT-2H 72.5 Hv-Consensus2006-Marcel-2H 72.01
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Map Data	Barley consensus 2003 Barley, Consensus 2006, DArT Barley, Consensus 2006, Marcel Barley, Steptoe x Morex, SSR
Probe	Bmag518
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Data Source	Langridge, Peter J.
Remark	Number of populations contributing to the placement of Bmag518 in the Barley, Consensus 2006 DArT maps = 2 Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : Steptoe x Morex
Possible Orthologs	Bmag0518
Probe	Bmag518
Locus	Bmag0518 Bmag518
Synonym	Other name Bmag0518
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (TC)23 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.66
Type	PCR SSR
PCR primers	5' AATGCCATGATGTTATTGG 3' 5' AAGAAGATTACATCGAATAGATCA 3' AATGCCATGATGTTATTGG AAGAAGATTACATCGAATAGATCA
SSR size	168
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare