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GrainGenes Marker Report: Bmag571

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Locus	Bmag571
Type	Microsatellite
Synonym	Synonym Bmag0571a Bmag0571b
Probe	Bmag571
Possible Orthologs	Bmag0571 Bmag0571a Bmag0571b Bmag0571c Bmag0571d
Probe	Bmag571
Locus	Bmag0571c Bmag0571a Bmag0571b Bmag571
Synonym	Other name Bmag0571
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (GA)25 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products Diversity Index (DI) = 0.64
Type	PCR SSR
PCR primers	5' AAGAACACCATATGATCCAAC 3' 5' ATTGGAGACGTATCATGTACC 3'
SSR size	154
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare